Search results for "Coulter counter"
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Comparison of a Manual and an Automated Method to Estimate the Number of Uterine Eggs in Anisakid Nematodes: To Coulter or Not to Coulter. Is That th…
2007
Studies reporting numbers of eggs in vagina and utero in nematodes often give little information of the technique used for the estimations. This situation hampers comparison among studies, because, so far, differences in estimations provided by different techniques have not been assessed. This note examines whether a manual method based on visual counts in aliquots and an automated method using a Coulter counter yield equivalent estimations of egg numbers in vagina and utero of 3 anisakid nematode species (Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum). The number of eggs from 50 females per nematode species was estimated using both techniques. The automated and ma…
Micromanipulation Techniques for the Isolation of Single Microorganisms
2005
A prerequisite for the biochemical and physiological investigation of microorganisms is the isolation and management of pure cultures. The only absolute criterion of purity for a bacterial culture is that it has been derived from the progeny of a single cell. Failure to apply this criterion may lead to much effort in proving the purity of a culture. All strains upon which research is to be based should therefore be rigorously purified before starting to investigate the properties of individual organisms (Johnstone 1969). Ecologically oriented microbiologists are faced especially with the problem of how to obtain a pure culture of certain microbial strains from their densely populated natura…